Natural or synthetic neopolyploids offer unique opportunities to quantify the immediate consequences of polyploidy. We developed a protocol based on seedling exposure to colchicine to obtain neotetraploids from wild diploid plants of Jasione maritima, with potential for being used in other species. High rates of tetraploid conversion were obtained, but survival decreased with increasing colchicine concentration. Therefore, low concentrations were ideal for obtaining neotetraploids.