Articles | Volume 18, issue 2
https://doi.org/10.5194/we-18-129-2018
https://doi.org/10.5194/we-18-129-2018
Standard article
 | Highlight paper
 | 
07 Sep 2018
Standard article | Highlight paper |  | 07 Sep 2018

Production of synthetic tetraploids as a tool for polyploid research

Mariana Castro, Sílvia Castro, and João Loureiro

Related subject area

Ecological Genetics
Validating the use of non-invasively sourced DNA for population genetic studies using pedigree data
Faye Wedrowicz, Jennifer Mosse, Wendy Wright, and Fiona E. Hogan
Web Ecol., 17, 9–18, https://doi.org/10.5194/we-17-9-2017,https://doi.org/10.5194/we-17-9-2017, 2017
Short summary

Cited articles

Allum, J. F., Bringloe, D. H., and Roberts, A. V.: Chromosome doubling in a Rosa rugosa Thunb. hybrid by exposure of in vitro nodes to oryzalin: the effects of node length, oryzalin concentration and exposure time, Plant Cell Rep., 26, 1977–1984, 2007. 
Ascough, G. D., Van Staden, J., and Erwin, J. E.: Effectiveness of colchicine and oryzalin at inducing polyploidy in Watsonia lepida NE Brown, HortScience, 43, 2248–2251, 2008. 
Barker, M. S., Arrigo, N., Baniaga, A. E., Li, Z., and Levin, D. A.: On the relative abundance of autopolyploids and allopolyploids, New Phytol., 210, 391–398, 2015. 
Bates, D., Maechler, M., Bolker, B., and Walker, S.: lme4: linear mixed-effects models using Eigen and S4, available at: http://CRAN.R-project.org/package=lme4 (last access: January 2018), 2014. 
Bretagnolle, F. and Lumaret, R.: Bilateral polyploidization in Dactylis glomerata L. subsp. lusitanica: occurrence, morphological and genetic characteristics of first polyploids, Euphytica, 84, 197–207, 1995. 
Download
Short summary
Natural or synthetic neopolyploids offer unique opportunities to quantify the immediate consequences of polyploidy. We developed a protocol based on seedling exposure to colchicine to obtain neotetraploids from wild diploid plants of Jasione maritima, with potential for being used in other species. High rates of tetraploid conversion were obtained, but survival decreased with increasing colchicine concentration. Therefore, low concentrations were ideal for obtaining neotetraploids.