The dispersal of soil nematodes was not affected by plant spatial configurations, which mostly varied according to their life-history strategy. However, creeping bentgrass grown in a more clustered spatial configuration developed a larger aboveground biomass, which was coupled with a reduction in biomass of subsequently grown ryegrass and plantain. The negative plant–soil feedback may be attributed to the depleted soil nutrients by the stimulated plant growth due to plant–nematode interactions.